Hybrid Embryonic Stem Cell-Derived Tetraploid Mice Show Apparently Normal Morphological, Physiological, and Neurological Characteristics
نویسندگان
چکیده
منابع مشابه
Generation of completely embryonic stem cell-derived mutant mice using tetraploid blastocyst injection
Embryonic stem (ES) cells provide a unique tool for producing specifically designed mutations in mice. Here, we describe an alternative approach toward the generation of mice which are derived completely from ES cells (ES mice), as judged by glucose phosphate isomerase (GPI) analysis, without prior passage through the germline. By injecting wild-type and mutant ES cells into tetraploid blastocy...
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The objective of this study was to generate transgenic mice expressing human resistin gene by using the tetraploidembryonic stem (ES) cell complementation method. Human resistin gene was amplified from human fetal liver cDNA library by PCR, cloned into pCR 2.1 TOPO vector and constructed in pCMV-Tag4C vector. Mammalian expression plasmid containing human resistin was transfected into D3-GL ES c...
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abstract regeneration is a biological phenomenon, which takes place via two main mechanisms: first, dedifferentiation of mature cells followed by their differentiation into functional new cells and second, activation of endogenous somatic stem cells for regeneration of damaged or lost tissues. one of the best examples of healing process in mammals is the regeneration of damaged pinna in rabbits...
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introduction: stem cell biology has been the subject of much recent discussion. embryonic stem (es) cells, derived from the inner cell mass of the blastocyst stage of early mammalian embryos are expected to become a powerful tool in future regenerative medicine and developmental biology due to their capacity of self-renewal and pluripotency. in the present study, the ultrastructural development...
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Polyploidy is occurred by the process of endomitosis or cell fusion and usually represent terminally differentiated stage. Their effects on the developmental process were mainly investigated in the amphibian and fishes, and only observed in some rodents as mammalian model. Recently, we have established tetraploidy somatic cell nuclear transfer-derived human embryonic stem cells (SCNT-hESCs) and...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 2003
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.23.11.3982-3989.2003